50S ribosomal subunit
  Woolford Laboratory  

Department of Biological Sciences l Carnegie Mellon University


Our laboratory studies the assembly of ribosomes, the complex ribonucleoprotein nanomachines that translate the genetic code and catalyze protein synthesis in all cells.  In eukaryotes, each ribosome contains 79 proteins and more than 5400  nucleotides in four RNA molecules. We use the yeast Saccharomyces cerevisiae as our model organism to investigate the pathway of large ribosomal subunit (LSU) biogenesis in eukaryotes. Yeast offers facile molecular genetic manipulation and analysis of the genome, as well as analysis of assembling ribosomes by proteomics, high throughput RNA structure probing, and high-resolution cryo-EM of structure. (more)



Dr. John L. Woolford, Jr. 
Office: Mellon Institute 618
Lab: Mellon Institute 618
Tel: (412) 268-3193 / Lab: (412) 268-3412
Fax: (412) 268-7129
Department of Biological Sciences
Carnegie Mellon University
4400 5th Avenue
Pittsburgh, PA 15213

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